Yep, I refreshed the usergroups, in fact I did it several times. Before I closed the computer, I double checked the usergroup settings, I do this each time I refresh the usergroups from vbulletin. Everything was at 200kb file size.
However, after closing the computer, and logging back in this morning, all the file size settings for several usergroups had reverted back to "0". This has happened on other occasions, and I find I am constantly having to double check my settings in the usergroups.